fertility training center chennai

TRAINING IN ICSI FREEZING & THAWING (SPERMS/EGGS/EMBRYOS)

  1. Intracytoplasmic sperm injection: Indications, location of ICSI set up, Choice of micro tools, microinjection, procedure and equipment, Intracytoplasmic morphologically selected sperm injection, Automation of ICSI
  2. Analysis of Fertilization: The physiology of fertilization, Fertilization in ART, Noninvasive evaluation of Zygote, Abnormal fertilization, assessment technique
  3. Morphological assessment of Oocyte and Embryo: Oocyte, Zygote, Early cleavage, Cleavage stage embryos
  4. Blastocyst culture and transfer: Why culture blastocyst till the blastocyst stage in vitro, Morphological evaluation of blastocysts, Factors influencing blastocyst development, Advantages and Applications of blastocyst culture, limitations of blastocyst transfer, Obstetric and perinatal outcome
  5. Advantages and disadvantages of Embryo transfer on Day 2, Day 3 and Blastocyst stage
  6. Embryo transfer technique: Pre-embryo transfer, Procedure of embryo transfer, trouble shooting, preventing an ectopic pregnancy, variations of technique
  7. Cryopreservation of oocytes and embryos by slow freezing method: Historical aspects, need for oocyte cryopreservation, advantages of embryo freezing, cryobiological principles of slow freezing, selection of embryos for freezing, Role of assisted hatching in Frozen embryos, rules for freezing and thawing, slow cooling of human oocytes, safety of oocyte and embryo freezing
  8. Oocyte vitification: Medical indications of Oocyte freezing, cryobiology-vitrification, Cryotools, With or without cumulus cells, Temperature at vitrification, Oocyte cryopreservation induced stress, Disease transmission issues, protocols for vitrification and warming.
  9. Vitrification: principles, various factors determining ultimate survival, Closed systems
  10. Embryo culture systems: culture media, culture conditions, media storage and equilibrium, incubators, Air purification unit, Quality control
  11. Micromanipulators and microinjections
  12. Introduction to cryobiology
  13. Cryopreservation media techniques
  14. Slow freezing methods, Equilibrium cooling, Vitrification methods, Non equilibrium cooling, Cryoinjuries, Cryoprotectants, Relevance of Cryopreservation
  15. Cryostorage vessels and carriers, Developmental Cell stage and embryo cryopreservation outcomes
  16. Zona Opening and/or thinning
  17. Endometrial preparation in Frozen Thawed cycles
  18. Safety of embryo cryopreservation
  19. Semen banking and effect of cryopreservation on sperm DNA
  20. Oocyte freezing and ovarian cortex cryopreservation
  21. Cryoprotectant free sperm vitrification for IVF/ICSI- A Novel Technology
  22. Making IVF safe with Vitrification.

Cryopreservation of ovarian tissue and ovarian cortex in Cancer patients

Duration:15 days

Course Fee:NA

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ADDRESS

Egmore, Chennai
Commander in Chief Road Ethirajsamy Salai, Next to Raddison Blu Egmore, Chennai-600 008

E-MAIL

support@fertilitytraining.org

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